P-116: Maturation Genes Expression during In Vitro Culture of Vitrified Mouse Preantral Follicles
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Abstract:
Background: Evaluation of maturation genes expression and their pattern during in vitro culture of vitrified preantral follicles is an essential and could promote either vitrification procedure or culture condition. Materials and Methods: Preantral follicles (with 100- 130 μm diameter) isolated mechanically from 12-14 days old NMRI mice ovaries and divided into vitrification and control groups. In the vitrification group, follicles were vitrified in a combination of ethylene glycol and dimethylsulfoxide, loaded on cryotop tip and immediately immersed in liquid nitrogen. Vitrified-warmed and fresh control follicles were cultured for 13 days and quantitative expressions of GDF9 and BMP15 as maturation genes and also BMPRII, ALK5, ALK6 as their receptors were assessed by real- time PCR after 3 hours, 4, 8 and 13 days of in vitro culture. Results: The results indicated that the expression of GDF9 and BMP15 in 3 hours and 4 days culture was increased in the follicles of vitrification group compared to the control group and also the expression of BMPRII rose after 4 and 8 days of culture, but with further culture up to 13 days the expression of these three genes were similar to control group. Additionally, the expression of maturation genes receptors such as ALK5 and ALK6 were similar in control and vitrification groups in all of assessed culture days. Conclusion: Although the expression rate of maturation genes was different in vitrification and control groups' follicles in some of the earliest days of culture, but in general the expression pattern of vitrification group was similar to control group during in vitro culture days.
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Journal title
volume 6 issue 2
pages -
publication date 2012-09-01
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